- Granny (Daisy May Moses)
- Called "Granny" by all, relatives or not, shotgun-toting Daisy Moses, Jed's mother-in-law, is a true daughter of Dixie. Paul Henning, the show's creator/producer quickly disposed of the idea of Granny being Jed's mother, which would have changed the show's dynamics, making Granny the matriarch and Jed subordinate to her. Granny can be aggressive but is often over-ruled by Jed. She is a confederate to the core, defending President Jefferson Davis, the Stars and Bars, and the simple life. Short-fused and easily angered, Granny fancies herself a "dunked" (not "sprinkled") Christian with forgiveness in her heart. She abhors "revenuers" and blue-coat Yankees. A self-styled "M.D." — "mountain doctor" — she claims to have an edge over expensive know-nothing city physicians. In lieu of anesthesia, Granny uses her "white lightning" brew before commencing on painful treatments such as leech bleeding and yanking teeth with pliers.
- Short and scrappy, Granny often wields a double-barreled, 12-gauge shotgun and fires it numerous times during the run of the show (in a first-season episode she chases Milburn Drysale with it when she found out his family had a feud with her family back in the hills). She fires it once at the front lawn when Jed is witching for water and several times on the skeet shooting range. During the mock Indian invasion she believed she was shooting live shells, though Milburn Drysdale had removed the buckshot to protect the actors portraying the Indians. She fires rock salt and bacon rind at a crow during the "Happy Valley" episode, and again at the back of an armored truck in which Milburn Drysdale was taking refuge in. She fires at (and hits in the posterior) Milburn Drysdale with rocksalt believing he is the ghost of "Lady Clemintine" ending their second visit to "Clampett Castle" in Kent, England.
- Granny also fires "Lady Fingers" (which Elly had baked for Jethro to take to the Army Reserve) into the posterior of an actor portraying Gen. Ulysses S. Grant during "The Battle of Culpepper Plantation"
- She is also able to tell the precise time via a sun dial and the weather via a beetle (Granny Versus the Weather Bureau). Without her glasses, Granny is extremely nearsighted — once in a crossover with the Petticoat Junction show, Granny mistakes a dog for a baby child and a coffee pot for a telephone. Two of Granny's phobias are "Injuns" (she actually buys wigs so the Clampetts won't be "scalped") and the "cement pond" (swimming pool–she has a fear of water). In a long story arc in the show's ninth season, Elly May dates a U.S. Navy frogman, which confuses Granny: After seeing the frogman climb out of the pool in his skin-diving wear, she thinks that anyone who swims in the pool will be turned into a frog. She also has a peculiar way of retelling the War Between The States, in which she thinks the South has won and Jefferson Davis is the president, while calling Sherman's March "Sherman's Retreat to the Sea". Any attempts to correct her meet with failure. She is also known for slicing off switches to use on Jethro, mainly whenever he goes too far with his dumb and idiotic schemes.
- There are references to Granny growing up in the Smoky Mountains of Tennessee. From episode 9: "When I was a girl back in Tennessee, I set so many boys' hearts on fire that they took to calling that neck of the woods The Smoky Mountains."
- Granny's full name, Daisy Moses, allegedly an homage to the popular and dearly loved folk artist Anna Mary Robertson, known to the world as Grandma Moses. (Grandma Moses died in 1961, a year before The Beverly Hillbillies made its television debut.) Granny is frequently referred to as "Granny Clampett" in a number of episodes but technically she is a Moses. Granny appears in all 274 episodes.
- The Daisy Moses (Granny) 1993 version.